|Year : 2016 | Volume
| Issue : 3 | Page : 169-174
The relationship between mean platelet volume and coronary collateral vessels in patients with acute coronary syndromes
Gaurav Singhal, Vijay Pathak
Department of Cardiology, SMS Hospital, Jaipur, Rajasthan, India
|Date of Web Publication||2-Mar-2017|
Assistant Professor, Department of Cardiology, SMS Hospital, Jaipur, Rajasthan
Source of Support: None, Conflict of Interest: None
Background: Elevated mean platelet volume (MPV) has been proposed as a risk factor for coronary artery disease (CAD) and is associated with poor clinical outcome in acute coronary syndrome (ACS). However, some studies have contradictory findings. Hence, we aimed to evaluate the association of MPV with the presence of coronary collateral vessel (CCV) in patients with ACS. Objective: To find MPV value in ACS patients and to find the predictive value of MPV in the spectrum of CAD and to examine whether levels of MPV predict the presence of CCVs. Methods: A total of 180 patients with first ACS were included in the study. MPV was measured. All patients underwent coronary angiography to know disease severity and CCVs. The CCVs are graded according to the Rentrop scoring system and according to coronary angiography results; patients were divided into two groups as Group 1 (poor CCV) and Group 2 (good CCV). Results: The MPV was 10.74 ± 2 fl in poor collateral group patients and 11.01 ± 1.7 fl in good collateral group (P = 0.421). The presence of CCV was not significantly associated with high levels of MPV. MPV value did not show any prediction of the spectrum of CAD. Conclusion: MPV on admission was not associated with the development of CCV positively in patients with ACS. Furthermore, it is not associated with a number of vessel involvements.
Keywords: Acute coronary syndrome, coronary artery disease, coronary collateral vessels, mean platelet volume, Rentrop criteria
|How to cite this article:|
Singhal G, Pathak V. The relationship between mean platelet volume and coronary collateral vessels in patients with acute coronary syndromes. J Pract Cardiovasc Sci 2016;2:169-74
|How to cite this URL:|
Singhal G, Pathak V. The relationship between mean platelet volume and coronary collateral vessels in patients with acute coronary syndromes. J Pract Cardiovasc Sci [serial online] 2016 [cited 2021 Apr 22];2:169-74. Available from: https://www.j-pcs.org/text.asp?2016/2/3/169/201374
| Introduction|| |
Acute coronary syndromes (ACS) are a set of signs and symptoms due to the rupture of a plaque and are a consequence of platelet-rich coronary thrombus formation. The thrombus leads to partial or complete coronary artery occlusion, which, in turn, leads to myocardial ischemia and various clinical manifestations ranging from unstable angina (UA) to acute myocardial infarction. Platelets play an important role in the pathophysiology of atherosclerosis and prothrombotic events leading to ACS. Platelets are heterogeneous with respect to their size and reactivity. Large platelets are metabolically and enzymatically more active than small ones and have higher thrombotic potential. Generalized platelet activation occurs during the acute coronary event, where the increase rate of platelet consumption at the site of atherosclerotic plaque rupture leads to the release of large size platelets from the bone marrow. This activation process results in signaling pathways that induce platelets to change their shape (metamorphosis) and size and become more active in secreting thromboxane A2 and adenosine diphosphate (ADP) into the circulation. Larger platelets are more adhesive and tend to aggregate more than smaller ones that contain more secretory granules and mitochondria and are known to be more active than smaller platelets. The release of thromboxane A2 and ADP also stimulates the neighboring platelets, causing them to become activated, and in turn secrete additional thromboxane A2 and ADP. Activated large size platelets directly bind to the circulating coagulation protein fibrinogen, through the abundant platelet integrin, glycoprotein IIb/IIIa receptors. The platelet–fibrinogen–platelet connection initiates the process of platelet aggregation and thus leads to coronary thrombus formation.
Mean platelet volume (MPV) is a useful biomarker of platelet activity.,, Increase in MPV is associated with poor clinical outcome and impaired angiographic reperfusion in patients with myocardial infarction (MI). Therefore, MPV is a marker of platelet reactivity. Unlike all other markers of platelet activation and reactivity, it is automatically calculated by most equipment for performing blood cell count. Thus, platelet size determination through MPV is a simple, extremely inexpensive, and readily available measure in hospital and outpatient settings.
Coronary collateral vessels (CCVs) circulation is a compensatory response to overcome injury in cases of severe ischemia secondary to tight stenosis or occluded vessels. Well-developed coronary collaterals can provide a perfusion reserve in case of increased myocardial oxygen demand and preserve ventricular function and viability, providing an alternative blood supply to an ischemic region in coronary artery disease (CAD). Prominent interindividual variability exists even among patients with similar degree of CAD. However, it remains unclear which factors are responsible for these differences. Several studies have shown that the development of collateral circulations (CCs) is impaired in the presence of many of the risk factors predisposing to atherosclerosis such as age, hypercholesterolemia, diabetes mellitus (DM), and smoking.,,,, In addition, many serum biomarkers, such as high-sensitivity C-reactive protein (CRP), lipoprotein-associated phospholipase A2, paraoxonase activity, and asymmetric dimethylarginine, have been reported to be associated with the development of CCs.,,,
The presence of CCV is correlated with well clinical outcomes in patients with ACS. Although many studies have reported the role of MPV in various cardiovascular diseases, some studies suggested that increased MPV predicted the risk of CAD and cardiac mortality. Hence, our interest is whether increased MPV predicts the presence of collaterals, which is also a major predictor of mortality in ACS. Association of higher MPV values with collaterals in ACS has been mostly studied among western patients. A few reports have revealed larger MPV values in Indian patients with ACS,, but no association of MPV with coronary collaterals is studied till now in Indian patients. In the present study, our aim was to find MPV value in ACS patients and to find the predictive value of MPV in the spectrum of CAD and to examine whether levels of MPV predict the presence of CCVs, one of the major predictors of mortality in patients with ACS.
| Methods|| |
A total of 180 consecutive patients who were admitted to hospital with the first episode of ACS were prospectively included in the study. Patients who underwent coronary angiography at the Department of Cardiology, SMS Hospital, Jaipur, between January 2015 and June 2015 were evaluated. Demographic and clinical data were obtained from the patients' medical records and detailed history. DM was defined as a history of DM, the use of antidiabetic drugs, or fasting plasma glucose levels of ≥7 mmol/L. Hypertension (HTN) was defined as a history of HTN or use of antihypertensive drugs or a blood pressure ≥140/90 mmHg. Smoking status was defined as current smoking. The study protocol was approved by the local Ethics Committee. Patients with hepatic or renal dysfunction (serum creatinine level >2 mg/dl), previous ACS patients, previous revascularization (coronary artery bypass graft operation or percutaneous coronary intervention) history, severe valvular disease, myocardial or pericardial disease, and technically inadequate coronary angiography were excluded from the study. Patients who were diagnosed with the hematological disease, cancer, systemic inflammatory or autoimmune disease, thrombocytopenia, and the use of anticoagulant agents were also excluded from the present study.
The sample size was calculated to allow detection of a 30% difference in MPV between different groups and with α of 0.05 and power of 80%. A total of 180 participants were recruited and studied in two groups. According to coronary angiography results, patients were divided into two groups as Group 1 (poor CCV) and Group 2 (good CCV). Informed consent was obtained from all patients. The study was approved by our local Ethics Committee.
In all cases, venous blood samples were drawn at admission before starting any medication. Serum glucose, blood urea, and serum creatinine were measured by standard methods. Tripotassium ethylenediaminetetraacetic acid-based anticoagulated blood samples were drawn and assessed using the Beckman Coulter LH 750 and Hmx systems (Beckman Coulter, USA) using original reagents. Hemoglobin, platelet count, white blood cell count, and MPV were analyzed using a blood counter (Sysmex 4000 I, Roche, Mannheim, Germany) that measures platelet size using aperture-impedance technology. All patient samples were processed within 2 h after venipuncture as recommended in the literature  to avoid bias due to excessive platelet swelling. The expected values for MPV in our laboratory ranged from 7.0–10.5 fl. A high MPV was defined as a value ≥10.5 fl.
Angiographic evaluation and collateral circulation grading
Coronary angiography was performed using standard techniques (Siemens Axiom Artis zee, Siemens Healthcare, Erlangen, Germany). Coronary angiography was performed through the femoral artery or radial artery for all patients using the Judkins technique. Each angiogram was interpreted by two experienced cardiologists who were blinded to the clinical details and results of the other investigations of each patient. CCVs were graded according to the Rentrop grading system of 0–3: 0 = No filling of any collateral vessel; 1 = Filling of the side branches of the artery to be perfused by collateral vessels without visualization of the epicardial segment; 2 = Partial filling of the distal epicardial segment by collateral vessels; and 3 = Complete filling of the distal epicardial segment by collateral vessels. The vessel that had the highest Rentrop grade with collaterals was used for analysis when more than one occluded vessel was present. In cases, for which more than one collateral vessel to the same occluded vessel was present, the highest Rentrop grade was used. Rentrop grade 0 accepted as no development of CCV, and Rentrop grade ≥1 was accepted as the presence of CCV. The study population was divided into two groups according to the Rentrop collateral score. Patients with grade 0–1 collateral development were classified as the poor collateral group; and patients with Rentrop grade 2–3 collateral development were classified as the good collateral group.
All analyses were performed using SPSS version 16.0 for windows (version 16.0, SPSS, Chicago, IL, USA). Quantitative variables were expressed as mean value ± standard deviation comparison of quantitative variables between two groups was performed by means of independent samples t-test. Categorical variables were compared by Chi-square test. A P < 0.05 was considered statistically significant.
| Results|| |
[Table 1] shows baseline clinical characteristics of the patients. [Table 2] and [Table 3] show the relation between the presence of CCV and baseline clinical and laboratory characteristics of the patients. The mean platelet count was 218.6 ± 98.4 (×103) in poor collateral group patients and 206.3 ± 76.53 (103) in good collateral group patients (P = 0.447). There was also no correlation between platelet counts with MPV. The MPV was 10.74 ± 2 fl in poor collateral group patients and 11.01 ± 1.7 fl in good collateral group (P = 0.421). The presence of CCV was not significantly associated with high levels of MPV (P = 0.421) [Table 3]. Furthermore, MPV value did not show any prediction of the spectrum of CAD. MPV was not significantly different in the number of vessel involvement (single vessel disease vs. double vessel disease vs. triple vessel disease). Mean age was 58.34 ± 11.04 years in poor collateral group and 57.36 ± 8.5 in good collateral group. Rentrop 0, 1, 2, and 3 were determined [Table 4] and [Figure 1] 102 (56.66%), 34 (18.88%), 30 (16.66%), and 14 (7.77%) patients, respectively. Rentrop 0–1 (poor collateral group) were determined in 136 (75.5%) patients. There was no significant difference in MPV value in Rentrop score. MPV value was 11.33 ± 1.23 in normal coronary patients, while in CAD patients, MPV value was 10.77 ± 1.96 (P = 0.325). MPV value was not different in diabetic patients from nondiabetic patients.
|Table 4: Number of patients according to Rentrop grade and mean platelet volume value of patients|
Click here to view
|Figure 1: Bar diagram showing number of patients according to Rentrop grade.|
Click here to view
| Discussion|| |
Previous studies showed effect of platelet volume on coronary adverse outcomes.,,, Well-developed CCs are important compensatory mechanisms in the protection of myocardium in the ischemic region during coronary occlusion. The factors involved in the development of CCs have been investigated in numerous studies. Several demographic, clinical, and biochemical factors have been reported to be associated with the degree of CC development.,,,,,,,, However, there are insufficient data regarding the relation between MPV and CCV. Well-developed CCVs can limit myocardial ischemia, may minimize the infarct size, and can protect the viable myocardium in patients with ACS.,,, The absence of CCV is correlated with bad clinical outcomes in patients with ACS. Resting distal coronary pressure consistently falls as stenosis severity exceeds 70%. Although the underlying mechanism remains uncharacterized, myocardial ischemia, growth factors (such as fibroblast growth factor (FGF) and vascular endothelial growth factor), various cells such as endothelial, monocytes, smooth muscle, cytokines, and shear stress are likely factors that contribute to initiating and remodeling for collateral development during tissue hypoxia.,,, Endothelial dysfunction is associated with poorer collateral growth. Furthermore, duration of angina pectoris and the extent of CAD are well-established determinants of CCVs.
Platelets play a key role in the pathogenesis, morbidity, and mortality of ACS. Increase in platelet activation is associated with CAD, carotid artery disease, and transplant vasculopathy.,,, Elevated MPV has been recognized as an independent risk factor for MI. Large platelets are metabolically and enzymatically more active than smaller ones and secrete and express more mediators such as adhesive proteins (fibrinogen, thrombospondin, and fibronectin), growth factors (platelet-derived growth factor, transforming growth factor-β, and basic FGF), and chemotactic and mitogenic factors (platelet factor 4, coagulation factors [factor V and factor XI], and cytokine-like factors [interleukin-1 and CD40 ligand]).,, Chemokines that are secreted from active platelets also stimulate smooth muscle cells and contribute to inflammation and atherogenesis. Inflammatory markers may also contribute to the absence of CCV through endothelial dysfunction. However, some studies showed that high MPV on admission was associated with the presence of CCV in patients with ACS., However, some other studies suggest opposite effect., Recently, Ege et al. reported that MPV levels were significantly higher, and Gensini score was significantly lower in the patients who had inadequate CCV in patients with CAD. In another study, high MPV levels were associated with severe CAD but not associated with the development of coronary collaterals. In our study, we found that high levels of MPV were not associated with the presence of CCV in patients with ACS.
Previous reports showed that there is a close relationship between MPV and other prognostic factors, such as smoking, diabetes, and HTN., However, our study showed no clear association between MPV and known ischemic heart disease risk factors. We did not find any association between development of CCV and age, HTN, smoking, or gender. In our study, there were not a sufficient number of patients with risk factors to assess the impact of these factors on the presence of collaterals. Thus, it is not possible to determine the effects of prognostic factors on the presence of collaterals according to the results of this study. Although the mechanism is still unclear, the increased megakaryocyte ploidy is positively, and a number of megakaryocytes are negatively correlated with platelet volume. Our study showed no association between elevated MPV and the total platelet count. Our study group includes only patients with ACS. We know that collaterals take the time to develop, but some studies showed early angiographic evidence of CC in patients with acute MI  and non-ST elevation MI. In our study, MPV was not significantly different in UA from non-ST elevation MI or ST elevation MI. We did not find any correlation between coronary collaterals and ACS presentation. Patients with ACS and a well-developed CC may have profited from a gradually developing stenosis through slow growth of mural thrombi. Finally, different methods can be used to analyze platelet activation. All tests have some limitations. Thus, MPV may provide an important, simple, effortless, and cost-effective marker which can be useful for evaluation of platelet activation, but it is not predictive of the development of coronary collaterals. Nonetheless, conflicting results of other studies make this issue controversial, which warrants performing of more comprehensive studies in future.
| Conclusion|| |
MPV on admission was not associated with the development of CCV positively in patients with ACS. Furthermore, it is not associated with a number of vessel involvements.
The present study had some limitations. First, the study population was relatively small. Second, inflammatory markers such as CRP and interleukin-6 were not analyzed. We did not quantify and compare CCs with invasive parameters other than the Rentrop scoring system. Patients with noncardiac chest pain (atypical symptoms) might have UA in the absence of electrocardiography changes and cardiac troponin positivity, have resulted in selection bias. Furthermore, there are no data about the physical activity of the patients, which encourages the development of CCVs. Angiographic techniques do not demonstrate vessels, of which the luminal diameter is 1.0 mm or less. Finally, individuals were enrolled from a single interventional cardiology center.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Broadley AJ, Gapper P, Schmitt M, Frenneaux MP. Supine rest reduces platelet activation and aggregation. Platelets 2003;14:3-7.
Tsiara S, Elisaf M, Jagroop IA, Mikhailidis DP. Platelets as predictors of vascular risk: Is there a practical index of platelet activity? Clin Appl Thromb Hemost 2003;9:177-90.
Corash L, Tan H, Gralnick HR. Heterogeneity of human whole blood platelet subpopulations. I. Relationship between buoyant density, cell volume, and ultrastructure. Blood 1977;49:71-87.
Huczek Z, Kochman J, Filipiak KJ, Horszczaruk GJ, Grabowski M, Piatkowski R, et al.
Mean platelet volume on admission predicts impaired reperfusion and long-term mortality in acute myocardial infarction treated with primary percutaneous coronary intervention. J Am Coll Cardiol 2005;46:284-90.
Pohl T, Seiler C, Billinger M, Herren E, Wustmann K, Mehta H, et al.
Frequency distribution of collateral flow and factors influencing collateral channel development. Functional collateral channel measurement in 450 patients with coronary artery disease. J Am Coll Cardiol 2001;38:1872-8.
Lambiase PD, Marber MS. Factors determining heterogeneity in coronary collateral development: A clinical perspective. Exp Clin Cardiol 2002;7:120-7.
Rivard A, Fabre JE, Silver M, Chen D, Murohara T, Kearney M, et al.
Age-dependent impairment of angiogenesis. Circulation 1999;99:111-20.
Van Belle E, Rivard A, Chen D, Silver M, Bunting S, Ferrara N, et al.
Hypercholesterolemia attenuates angiogenesis but does not preclude augmentation by angiogenic cytokines. Circulation 1997;96:2667-74.
Weihrauch D, Lohr NL, Mraovic B, Ludwig LM, Chilian WM, Pagel PS, et al.
Chronic hyperglycemia attenuates coronary collateral development and impairs proliferative properties of myocardial interstitial fluid by production of angiostatin. Circulation 2004;109:2343-8.
Tatli E, Altun A, Büyüklü M, Barotçu A. Coronary collateral vessel development after acute myocardial infarction. Exp Clin Cardiol 2007;12:97-9.
Koerselman J, de Jaegere PP, Verhaar MC, Grobbee DE, van der Graaf Y; SMART Study Group. Coronary collateral circulation: The effects of smoking and alcohol. Atherosclerosis 2007;191:191-8.
Kerner A, Gruberg L, Goldberg A, Roguin A, Lavie P, Lavie L, et al.
Relation of C-reactive protein to coronary collaterals in patients with stable angina pectoris and coronary artery disease. Am J Cardiol 2007;99:509-12.
Orem C, Kahraman N, Orem A, Uçar U, Yucesan FB, Mentese A. Increased plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) levels are related to good collateral development in patients with isolated left coronary artery disease. Int J Cardiol 2011;148:117-9.
Yildiz A, Sezen Y, Gur M, Yilmaz R, Demirbag R, Erel O. Association of paraoxonase activity and coronary collateral flow. Coron Artery Dis 2008;19:441-7.
Kocaman SA, Sahinarslan A, Biberoglu G, Hasanoglu A, Akyel A, Timurkaynak T, et al.
Asymmetric dimethylarginine and coronary collateral vessel development. Coron Artery Dis 2008;19:469-74.
Prêtre R, Rickli H, Ye Q, Benedikt P, Turina MI. Frequency of collateral blood flow in the infarct-related coronary artery in rupture of the ventricular septum after acute myocardial infarction. Am J Cardiol 2000;85:497-9.
Mukamal KJ, Wellenius GA, Mittleman MA. Hematologic parameters, atherosclerotic progression, and prognosis in patients with previous coronary artery bypass grafting (from the Post CABG Trial). Am J Cardiol 2009;103:328-32.
De Luca G, Santagostino M, Secco GG, Cassetti E, Giuliani L, Franchi E, et al.
Mean platelet volume and the extent of coronary artery disease: Results from a large prospective study. Atherosclerosis 2009;206:292-7.
Ranjith MP, Divya R, Mehta VK, Krishnan MG, KamalRaj R, Kavishwar A. Significance of platelet volume indices and platelet count in ischaemic heart disease. J Clin Pathol 2009;62:830-3.
Demirtunc R, Duman D, Basar M. Effects of doxazosin and amlodipine on mean platelet volume and serum serotonin level in patients with metabolic syndrome: A randomised, controlled study. Clin Drug Investig 2007;27:435-41.
Pal R, Bagarhatta R, Gulati S, Rathore M, Sharma N. Mean platelet volume in patients with acute coronary syndromes: A supportive diagnostic predictor. J Clin Diagn Res 2014;8:MC01-4.
Rentrop KP, Cohen M, Blanke H, Phillips RA. Changes in collateral channel filling immediately after controlled coronary artery occlusion by an angioplasty balloon in human subjects. J Am Coll Cardiol 1985;5:587-92.
Bonow RO, Mann DL, Zipes DP, Libby P. Braunwald's Heart Disease: A Textbook of Cardiovascular Medicine. Part VII. 9th
ed. Philadelphia, PA: Saunders; 2012. p. 1064-6.
Arslan U, Kocaoglu I, Balci M, Duyuler S, Korkmaz A. The association between impaired collateral circulation and non-alcoholic fatty liver in patients with severe coronary artery disease. J Cardiol 2012;60:210-4.
Van Royen N, Piek JJ, Schaper W, Bode C, Buschmann I. Arteriogenesis: Mechanisms and modulation of collateral artery development. J Nucl Cardiol 2001;8:687-93.
Matsunaga T, Warltier DC, Weihrauch DW, Moniz M, Tessmer J, Chilian WM. Ischemia-induced coronary collateral growth is dependent on vascular endothelial growth factor and nitric oxide. Circulation 2000;102:3098-103.
Seiler C. The human coronary collateral circulation. Heart 2003;89:1352-7.
Abaci A, Oguzhan A, Kahraman S, Eryol NK, Unal S, Arinç H, et al.
Effect of diabetes mellitus on formation of coronary collateral vessels. Circulation 1999;99:2239-42.
Murat SN, Duran M, Kalay N, Gunebakmaz O, Akpek M, Doger C, et al.
Relation between mean platelet volume and severity of atherosclerosis in patients with acute coronary syndromes. Angiology 2013;64:131-6.
Thaulow E, Erikssen J, Sandvik L, Stormorken H, Cohn PF. Blood platelet count and function are related to total and cardiovascular death in apparently healthy men. Circulation 1991;84:613-7.
Willoughby S, Holmes A, Loscalzo J. Platelets and cardiovascular disease. Eur J Cardiovasc Nurs 2002;1:273-88.
Fateh-Moghadam S, Bocksch W, Ruf A, Dickfeld T, Schartl M, Pogátsa-Murray G, et al.
Changes in surface expression of platelet membrane glycoproteins and progression of heart transplant vasculopathy. Circulation 2000;102:890-7.
Fateh-Moghadam S, Li Z, Ersel S, Reuter T, Htun P, Plöckinger U, et al.
Platelet degranulation is associated with progression of intima-media thickness of the common carotid artery in patients with diabetes mellitus type 2. Arterioscler Thromb Vasc Biol 2005;25:1299-303.
Elsherbiny IA, Shoukry A, El Tahlawi MA. Mean platelet volume and its relation to insulin resistance in non-diabetic patients with slow coronary flow. J Cardiol 2012;59:176-81.
Endler G, Klimesch A, Sunder-Plassmann H, Schillinger M, Exner M, Mannhalter C, et al.
Mean platelet volume is an independent risk factor for myocardial infarction but not for coronary artery disease. Br J Haematol 2002;117:399-404.
Kaya MG, Yarlioglues M, Gunebakmaz O, Gunturk E, Inanc T, Dogan A, et al.
Platelet activation and inflammatory response in patients with non-dipper hypertension. Atherosclerosis 2010;209:278-82.
Gawaz M, Langer H, May AE. Platelets in inflammation and atherogenesis. J Clin Invest 2005;115:3378-84.
Varol E, Akcay S, Ozaydin M, Erdogan D, Dogan A, Altinbas A. Mean platelet volume is associated with insulin resistance in non-obese, non-diabetic patients with coronary artery disease. J Cardiol 2010;56:154-8.
Gawaz M. Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium. Cardiovasc Res 2004;61:498-511.
Schneeweis C, Gräfe M, Bungenstock A, Spencer-Hänsch C, Fleck E, Goetze S. Chronic CRP-exposure inhibits VEGF-induced endothelial cell migration. J Atheroscler Thromb 2010;17:203-12.
Duran M, Gunebakmaz O, Uysal OK, Ocak A, Yilmaz Y, Arinc H, et al.
Relation between mean platelet volume and coronary collateral vessels in patients with acute coronary syndromes. J Cardiol 2013;61:295-8.
Ayhan S, Ozturk S, Erdem A, Ozlu MF, Memioglu T, Ozyasar M, et al.
Hematological parameters and coronary collateral circulation in patients with stable coronary artery disease. Exp Clin Cardiol 2013;18:e12-5.
Ege MR, Acikgoz S, Zorlu A, Sincer I, Guray Y, Guray U, et al.
Mean platelet volume: An important predictor of coronary collateral development. Platelets 2013;24:200-4.
Sani N, Mustafa D, Nihat K, Ozgur G. Relation between mean platlet volume and severity of atherosclerosis in patients with acute coronary syndrome. Angiology 2013;64:131-6.
Ege MR, Acıkgoz S, Zorlu A, Sıncer I, Guray Y, Guray U, et al
. Mean platelet volume in patients with type 2 diabetes mellitus. Platelets 2004;15:475-8.
Inanc T, Kaya MG, Yarlioglues M, Ardic I, Ozdogru I, Dogan A, et al.
The mean platelet volume in patients with non-dipper hypertension compared to dippers and normotensives. Blood Press 2010;19:81-5.
Elsman P, van 't Hof AW, de Boer MJ, Hoorntje JC, Suryapranata H, Dambrink JH, et al.
Role of collateral circulation in the acute phase of ST-segment-elevation myocardial infarction treated with primary coronary intervention. Eur Heart J 2004;25:854-8.
Bahrmann P, Rach J, Desch S, Schuler GC, Thiele H. Incidence and distribution of occluded culprit arteries and impact of coronary collaterals on outcome in patients with non-ST-segment elevation myocardial infarction and early invasive treatment strategy. Clin Res Cardiol 2011;100:457-67.
Haubelt H, Simon M, Anders CH, Hellstern P. Platelet function tests for monitoring of acetylsalicylic acid: Clinical significance in antiplatelet treatment. Hamostaseologie 2004;24:196-202.
[Table 1], [Table 2], [Table 3], [Table 4]