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  Indian J Med Microbiol
 

Figure 4: Comparative analysis of cardiac specific gene expression (a) Reverse transcription-polymerase chain reaction analysis showing comparative expression profile of cardiac markers in conditioned media and 5-azacytidine treated mesenchymal stem cells (lanes 1 and 2 respectively) and positive control cardiac muscle biopsy (lane 3) (b) graph of quantitative polymerase chain reaction experiment showing expression levels of (i) myosin light chain-2v (ii) sarcoplasmic calcium 2 + ATase (iii) ryonodine receptor 2 in conditioned media and 5-azacytidine induced mesenchymal stem cells relative to uninduced mesenchymal stem cell. 5-azacytidine treated cells showed higher expression of all markers as compared to conditioned media treated cells. Data represent means ± standard deviation of three independent experiments.

Figure 4: Comparative analysis of cardiac specific gene expression (a) Reverse transcription-polymerase chain reaction analysis showing comparative expression profile of cardiac markers in conditioned media and 5-azacytidine treated mesenchymal stem cells (lanes 1 and 2 respectively) and positive control cardiac muscle biopsy (lane 3)  (b) graph of quantitative polymerase chain reaction experiment showing expression levels of (i) myosin light chain-2v (ii) sarcoplasmic calcium 2 + ATase (iii) ryonodine receptor 2 in conditioned media and 5-azacytidine induced mesenchymal stem cells relative to uninduced mesenchymal stem cell. 5-azacytidine treated cells showed higher expression of all markers as compared to conditioned media treated cells. Data represent means ± standard deviation of three independent experiments.